Abstract
Purpose: To investigate the effect of “HuChou San” on axillary osmidrosis (AO) by measuring ABCC11 mRNA levels and MRP8 protein expression in axillary skin tissues from “HuChou San”-treated AO patients.Methods: Forty AO patients undergoing treatment at Shanghai Ninth People’s Hospital were enrolled in the study. Tissues from axillary regions were initially treated for 10 min with a solution of copper sulphate and zinc sulphate, followed by treatment with gentle application of “HuChou San” once daily for 3 months. Ten healthy individuals served as untreated control. Axillary skin tissue was surgically collected at 0, 1, 2, and 3 months of treatment. The expressions of ABCC11 mRNA and MRP8 protein in axillary skin samples were determined using qPCR and Western blot, respectively.Results: The qPCR and Western blot results showed higher levels of ABCC11 mRNA and MRP8 protein in AO patient tissues than in healthy controls. However, “HuChou San” treatment markedly reduced tissue levels of ABCC11 mRNA and MRP8 protein (p < 0.05), when compared to tissues extracted prior to treatment. These effects were time-dependent and increased as treatment duration increased (p < 0.05).Conclusion: “HuChou San” powder significantly and time-dependently reduced ABCC11 mRNA and MRP8 protein expressions in AO axillary tissue of AO patients. It is known that ABCC11 mRNA and MRP8 protein are two important indices of AO. Therefore, the findings of this study suggest that“HuChou San” has potentials for use as a novel alternative therapy for AO.
 Keywords: ABCC11 mRNA, MRP8 protein, Axillary osmidrosis, HuChou San
Highlights
Axillary osmidrosis (AO) is a clinical condition that manifests in an offensive and unpleasant body odour originating from the axillary area
One month of daily “HuChou San” treatment caused a significant decrease in MRP8 protein expression in AO patient tissue, relative to tissue taken before treatment (p < 0.05)
The ATP-binding cassette sub-family C member 11 (ABCC11) mRNA levels were significantly elevated in axillary tissue from untreated AO patients, when compared to healthy control tissues (p < 0.01)
Summary
Axillary osmidrosis (AO) is a clinical condition that manifests in an offensive and unpleasant body odour originating from the axillary area This odour, which is produced by secretions from the axillary apocrine glands, can cause significant distress and impair the psychosocial well-being of the affected individuals. The use of Several surgical techniques have been developed for removal of the axillary apocrine glands These procedures include removal of subcutaneous tissue, total excision of skin and subcutaneous tissue, ultrasonic liposuction, electrodessication, as well as sympathectomy. These surgical techniques have yielded variable results [5,6,7]. This prompted the present study aimed at further investigations on the efficacy of “HuChouSan”
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