Abstract
Ehrlich ascites carcinoma is a spontaneous murine mammary adenocarcinoma adapted to ascites form and carried in outbred mice by serial intraperitoneal (i/p) passages. The previous work from our laboratory showed that honey having higher phenolic content was potent in inhibiting colon cancer cell proliferation. In this work, we extended our research to screen the antitumor activity of two selected honey samples and eugenol (one of the phenolic constituents of honey) against murine Ehrlich ascites and solid carcinoma models. Honey containing higher phenolic content was found to significantly inhibit the growth of Ehrlich ascites carcinoma as compared to other samples. When honey containing higher phenolic content was given at 25% (volume/volume) intraperitoneally (i/p), the maximum tumor growth inhibition was found to be 39.98%. However, honey was found to be less potent in inhibiting the growth of Ehrlich solid carcinoma. On the other hand, eugenol at a dose of 100 mg/kg i/p was able to inhibit the growth of Ehrlich ascites by 28.88%. In case of solid carcinoma, eugenol (100 mg/kg; i/p) showed 24.35% tumor growth inhibition. This work will promote the development of honey and eugenol as promising candidates in cancer chemoprevention.
Highlights
The previous work from our laboratory showed that honey could induce apoptosis in colon cancer cell lines
Ehrlich ascites carcinoma (EAC) cells were collected from the ascitic fluid of BALB/c mice harbouring 8–10 days old ascitic tumor. 1 × 107 EAC cells were injected intraperitoneally in 52 BALB/c female mice selected for the experiment on day 0
Our previous studies reported that the apoptotic potential of honey varied according to the source and phenolic content [1, 2]
Summary
The previous work from our laboratory showed that honey could induce apoptosis in colon cancer cell lines. Four honey samples named as Sample A, B, C, and D of different origins were investigated for their phenolic content. Sample C showed higher phenolic content of 65.06 Gallic acid equivalent (GAE), per 100 g of honey followed by Sample A (60 GAE), Sample D (47.10 GAE) and Sample B (29.96 GAE). The apoptotic potential of honey against colon cancer cells was found to vary among the samples depending upon the phenolic content. Sample C showing higher phenolic content was found to be more potent in inhibiting the cancer cell proliferation as compared to other samples [1, 2]. We investigated the antitumor effect of two honey samples (Sample C possessing higher phenolic content and Sample B with lower phenolic content) as well as eugenol (one of the phenolic constituents of honey) against Ehrlich ascites and solid carcinoma
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