Effect of histone deacetylase inhibitor MGCD0103 on hepatocellular carcinoma cell line BEL7402 in vitro

Abstract

Objective To investigate the effect of histone deacetylase inhibitor MGCD0103 on the growth inhibition of hepatocellular carcinoma cell line BEL7402. Methods BEL7402 cells were treated with different concentrations (0, 0.1, 1.0, 5.0, 10.0, 50.0, 100.0 μmol/L) of MGCD0103 for 24, 48, or 72 h and their growth was tested by cell counting kit-8 (CCK-8) assay. Cell viability and half maximal inhibitory concentration were then calculated. Cell tumorigenicity of BEL7402 cells treated with MGCD0103 for 48 h was tested using colony formation assay. The distribution of cell cycle and the apoptosis rate of BEL7402 cells treated with MGCD0103 for 48 h were analyzed by flow cytometry. Results MGCD0103 inhibited the growth of BEL7402 cells in a dase-dependent manner. The half maximal inhibitory concentrations for MGCD0103 at 24, 48, and 72 h were (19.36±2.13), (9.12±0.87) and (4.95±0.45) μmol/L respectively. MGCD0103 attenuated cell tumorigenicity of BEL7402 cells. MGCD0103 influenced the cell cycle distribution of BEL7402 cells and caused G2/M phase arrest. After treatment, the proportion of G2/M phase cells was increased from (7.83±0.41)% to (15.84±1.69)% (P=0.001). MGCD0103 triggered apoptosis of BEL7402 cells. The apoptosis rate was (6.23±0.66)% (control group), (9.02±1.03)% (1 μmol/L) (P=0.017), (15.93±1.77)% (5 μmol/L) (P=0.001), and (35.30±2.68)% (20 μmol/L) (P=0.000), respectively. Conclusion MGCD0103 has inhibitory effect on the growth of BEL7402 cells. Key words: Histone deacetylase inhibitor; MGCD0103; Carcinoma, hepatocellular; Apoptosis