Abstract
Effects of inhibited antigravity activity of hindlimb muscles on the hippocampal neurogenesis were studied in newly weaned rats, using the hindlimb suspension (HS) model. Effects of ambulation recovery were also studied. Brain was fixed by infusion of phosphate buffer with 4% paraformaldehyde in the anesthetized rat and cross‐sectional samples, around the dentate gyrus (DG) of the hippocampus, were analyzed immunohistochemically. Proliferating cell nuclear antigen (PCNA) and doublecortin (DCX) were used as markers for mitotic cell and immature neuron, respectively. Both PCNA+ and DCX+ cells in DG decreased gradually following growth in unsuspended control rats. Decrease of DCX+ cells was further stimulated significantly by 14 days of HS, although the number of PCNA+ cells in the suspended rats was the same as controls. After 14 days of recovery, however, the number of DCX+ cells of the suspended rats returned to the control levels. These results suggest that HS transiently impaired hippocampal neurogenesis, especially in the differentiation of new‐born neurons. This study was supported by Grant‐in‐Aid for Scientific Research (19100009, 21700654) from Japan Society for the Promotion of Science.
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