Effect of high porosity on biodegradation of poly (4-hydroxybutyrate)in vivo

Abstract

Poly-4-hydroxybutyrate, P(4HB), is a biosynthetic thermoplastic polymer that has been studied as a bio-absorbable medical material. In order to explore the in vivo biodegradation behaviour of porous P(4HB) membranes with specified apertures (89-150 µm), membranes with different porosities were implanted subcutaneously into the backs of 27 eight-week-old Sprague Dawley® rats. The implanted specimens were examined with Masson and hematoxylin and eosin staining. Masson staining indicated that the P(4HB) membranes were encased in fibrous cysts and that more collagen fibers were present within the sections of the hyper-porosity group. Hematoxylin and eosin staining showed that the residual area of the P(4HB) membranes in the hyper-porosity group decreased sharply compared to the hypo-porosity group, which implied that the P(4HB) membranes with higher porosity degraded faster than those with lower porosity. A slow degradation phase persisted for approximately 14 weeks during the degradation process. After the 16th week, the P(4HB) scaffolds fell into a fast degradation phase. The residual areas of the hyper-porosity P(4HB) membranes at the 32nd week were reduced by 39.76% compared with the second week after implantation. We concluded that P(4HB) membranes manifest a special biodegradation behavior in vivo and that the increased porosity of these membranes is an important factor favoring their biodegradation rates.