Abstract
Objective To observe the effects of high mobility protein A2 (HMGA2) overexpression on proliferation and cell cycle of rat pituitary tumor cells.Methods The mRNA and protein expression of HMGA2 in rat GH3 cells transduced by pIRES2-dsRed-HMGA2cDNA were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting.The effects of the HMGA2 overexpression on GH3 cell proliferation and cell cycle were analyzed by cell counting kit-8 (CCK-8) assay and flow cytometry.Results pIRES2-dsRed-HMGA2cDNA transfected rat GH3 cells after 24 h the HMGA2 mRNA and protein expression levels were respectively higher than the control group 26.40％ and 36.60％,the difference was statistically significant (P ＜ 0.05).To compare with control group the number of pIRES2-dsRed-HMGA2cDNA group cells increased at 24,48,72,96 h (P ＜ 0.05).Flow cytometry analysis showed that the experimental group,the percentage of cells in G0/G1 phase was significantly reduced compared with the control group,(43.15 ±0.63)％ vs.(65.33 ±0.37)％,while the S phase was significantly increased compared with the control group (39.57 ± 0.65) ％ vs.(18.26 ± 0.54) ％ (P ＜ 0.05).Conclusion HMGA2 overexpression increases rat GH3 cell proliferation and changes normal cell cycle. Key words: High mobility protein A2; Over expression; Pituitary tumor; Proliferation; Cell cycle
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