Effect of high hydrostatic pressure extract of Korean ginseng on adipogenesis in 3T3‐L1 adipocytes (1045.4)

Abstract

Ginseng, especially steam‐and‐dry processed red ginseng, has been shown to decrease adipogenesis in adipose tissue. In the food processing industry, high hydrostatic pressure extraction technique has become an alternative to heat processing. The aim of this study was to comparatively investigate the anti‐adipogenic effects of water extract of red ginseng (WRG) and high hydrostatic pressure extract of fresh ginseng (HPG) in 3T3‐L1 adipocytes. Intracellular lipid and triglyceride were decreased in both WRG and HPG treated cells, while the cell viability remained unaffected. This observed decrease was consistent with the reduction of GPDH activity, a key enzyme in triglyceride biosynthesis. The mRNA expressions of adipogenic genes, including peroxisome proliferator‐activated receptor‐gamma, CCAAT/enhancer‐binding protein‐α, sterol regulatory element‐binding protein‐1c, and adipocyte fatty acid binding protein, were significantly decreased in both WEG and HPG treated cells. The activity of AMPK, a key regulator of energy metabolism, was significantly increased in the HPG treated cells compared to the WRG. From these results, it is assumed that anti‐adipogenic mechanisms of the WRG and HPG in adipocytes might be associated with inhibition of GPDH activity and adipogenic gene expression as well as AMPK activation. Moreover, efficiency of the HPG might be comparable to that of the WRG.Grant Funding Source: This work was supported by the Brain Korea 21 plus project funding (No. 22A20130012143)