Effect of high-fat diet on body mass and energy balance in the bank vole

Abstract

We tested the hypothesis that animals that reduce their body mass in response to decreased photoperiod do not develop diet-induced obesity (DIO) when fed a high-fat diet (HFD). Bank voles ( Clethrionomys glareolus) fed a diet with fat content of 13.5% by mass and 28.2% by energy, for 5 weeks, did not develop hyperphagia and were resistant to DIO, consistent with predictions. There was no significant difference between food (g/day) or energy intake (kJ/day) between the experimental or control group (fed a standard diet with fat content of 5.4% by mass and 12.3% by energy) over the duration of the experiment. However, as a result of a higher apparent energy assimilation efficiency (AEAE), voles fed the HFD assimilated significantly more energy over the 5-week period. Furthermore, they consumed twice as much fat per day as controls. There were no significant differences in resting metabolic rate (RMR) over time or between groups over the 5-week period. In conclusion, bank voles achieved resistance to DIO despite assimilating more energy than control animals fed the standard diet and taking in twice as much fat. Resistance did not occur by modification of RMR, implicating differences in activity levels.