Abstract
Objective To investigate the effect of high expression of protein tyrosine phosphatase SHP-1 on the tumor cell invasiveness and chemosensitivity of esophageal squamous cell carcinoma. Methods EC9706 cells of esophageal squamous cell carcinoma were divided into three groups: the blank group was not given any treatment; the experimental group used SHP-1 mimic instantaneous vector to transfect cells for 24 h, and used 10 μmol/L cisplatin to treat EC9706 cells for 12 h; the control group selected 10 μmol/L cisplatin to treat EC9706 cells for 12 h. Cell proliferation was detected by using methyl thiazolyl tetrazolium (MTT) assay, and cell invasion was detected by using Transwell chamber. The expression of SHP-1 mRNA was detected by using fluorescence quantitative polymerase chain reaction. The expression level of SHP-1 protein was detected by using Western blot. Results The expression of SHP-1 mRNA in the experimental group (3.42±0.14) was higher than that in the control group and the blank group (1.09±0.13, 0.42±0.24, F = 9.143, P < 0.05). Compared with the control group and the blank group, the cell growth inhibition rate and the apoptosis index in the experimental group were increased (both P < 0.05), and the differences between any two groups were also statistically significant (all P < 0.05). The cell invasion rate in the experimental group, the control group and the blank group was (6.5±1.3) %, (18.5±2.5) % and (45.2±7.2) %, respectively, and the difference was statistically significant (F = 11.853, P < 0.05). Conclusion High expression of SHP-1 can inhibit the invasion of esophageal squamous cell carcinoma, improve chemosensitivity, promote apoptosis and inhibit cell proliferation, which could lay a theoretical foundation for improving the efficacy of chemotherapy for esophageal squamous cell carcinoma. Key words: Esophageal neoplasms; Protein tyrosine phosphatase; SHP-1; Chemotherapy; Sensitivity
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