Effect of high altitude on human postprandial 13C‐octanoate metabolism, intermediary metabolites, gastrointestinal peptides, and visceral perception

Abstract

At high altitude (HA), acute mountain sickness (AMS) is accompanied by neurologic and upper gastrointestinal symptoms (UGS). The primary aim of this study was to test the hypothesis that delayed gastric emptying (GE), assessed by 13 C-octanoate breath testing (OBT), causes UGS in AMS. The secondary aim was to assess post-gastric mechanisms of OBT, which could confound results under these conditions, by determination of intermediary metabolites, gastrointestinal peptides, and basal metabolic rate. A prospective trial was performed in 25healthy participants (15male) at 4559m (HA) and at 490m (Zurich). GE was assessed by OBT (428kcal solid meal) and UGS by visual analogue scales (VAS). Blood sampling of metabolites (glucose, free fatty acids (FFA), triglycerides (TG), beta-hydroxyl butyrate (BHB), L-lactate) and gastrointestinal peptides (insulin, amylin, PYY, etc.) was performed as well as blood gas analysis and spirometry. variance analyses, bivariate correlation, and multilinear regression analysis. After 24h under hypoxic conditions at HA, participants developed AMS (p<0.001). 13 CO2 exhalation kinetics increased (p<0.05) resulting in reduced estimates of gastric half-emptying times (p<0.01). However, median resting respiratory quotients and plasma profiles of TG indicated that augmented beta-oxidation was the main predictor of accelerated 13 CO2 -generation under these conditions. Quantification of 13 C-octanoate oxidation by a breath test is sensitive to variation in metabolic (liver) function under hypoxic conditions. 13 C-breath testing using short-chain fatty acids is not reliable for measurement of gastric function at HA and should be considered critically in other severe hypoxic conditions, like sepsis or chronic lung disease.