Abstract
Background; Herbal medicine is the world's oldest type of healthcare. Hibiscus rosa flower and leave extracts are used as natural remedies to treat various diseases, and as a reversible antifertility for males. Aim and objectives; this study aimed to investigate the effectiveness of nanoparticles to improve the impact of Hibiscus rosa flower extract as an anti-fertility substance. Animals and methods; twenty male albino rats were divided into five groups: G1 (-ve control): treated with normal saline (0.2 ml per day), G2 (+ve control): treated with nanoparticles (300mg empty liposome per day), G3: treated with flower extract nanoparticles (100mg/kg per day), G4: treated with flower extract nanoparticles (200mg/kg per day), and last group G5: treated with flower extract nanoparticles (300mg/kg per day). Sperm function tests (sperm concentration, sperm motility percent, and sperm viability percent) were estimated after the treatment in the five groups after 55 days of daily treatment. Besides, the diagnosis of compounds in the extracts of palmic acid “ethyl ester”, ascorbic acid, 9-octadecenoic acid “methyl ester”, methyl stearate, oleic acid, 9,12-octadecadienoic acid, eicosane, heptadecane, 1-(1,5-dimethyl hexyl, fumaric acid, butanediol, stigmasterol, and beta-sitosterol, was performed using gas chromatography-mass (GC/MS) method.
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