EFFECT OF HEXANE EXTRACT OF Mikania glomerata AGAINST Biomphalaria glabrata AND Schistosoma mansoni

Abstract

Purpose: To evaluate the potential of the hexane extract of Mikania glomerata against adult snails and embryos of Biomphalaria glabrata, and in young and adult worms of Schistosoma mansoni . Methods: Adult snails and embryos were kept in chlorinated water and exposed to the extract at concentrations of 12.5; 25; 50 and 100 µg/mL for 24 hours. After exposure, they were washed and observed using a microscope for seven days for analysis of mortality and malformations. Juvenile and adult worms were recovered by perfusion of the portal-hepatic system mice, cultured in RPMI medium with different concentrations of the extract for 72 hours, and viability was assessed using an inverted microscope. Results : M. glomerata caused death in adult snails only at a concentration of 100 µg/mL and showed a Lethal Concentration (LC 50 ) value higher than 100 µg/mL. For embryos was observed a reduction the 97.2 and 100% in embryos development at concentrations of 25, 50 and 100 ppm, and it demonstrated a LC 50 value the 29.64 µg/mL. For juvenile worms also was observed a reduction in viability of the parasites and showed a LC 50 value the 23.80 µg/mL at 24 hours. In adult worms was noted that the extract of M. glomerata caused a reduction in the viability of parasites and the LC 50 value was the 67.39 µg/mL at 24 hours. Conclusion: Thus, this study opens perspectives for new studies in schistosomiasis control with natural products against B. glabrata and the life stages of the parasite S. mansoni .