Abstract

Effect of herbicides Afalon (linuron), Aretit (dinoseb acetate), Gesatop 50 (simazine) was tested concemitantly with two NPK fertilization levels (N - 0, P<sub>2</sub>O<sub>5</sub> - 36 kg/ha, K<sub>2</sub>O - 60 kg/ha and N - 70 kg/ha, P<sub>2</sub>O<sub>5</sub> - 72 kg/ha, K<sub>2</sub>O - 120 kg/ha) on the <i>ß</i>-carotene content of field bean leaves. The carotenoids content was determined by thin-layer chromatography on magnesium oxide in the system petroleum ether: acetone (88 : 12 v/v). An increase of <i>ß</i>-carotene content in the field bean leaves at the flower-bud formation stage was found, after application of above mentioned herbicides and high dosage NPK fertilization levels. No influence of herbicides and mineral fertilization on the (<i>ß</i>-carotene content in the plant leaves could be demonstrated at the full pods stage.

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