Abstract
Rat ventral prostate is very rich in polyamines that, during homogenization, cause aggregation of subcellular organelles jeopardizing subfractionation studies of this organ. 1. 1) Heparin added in vitro to rat ventral prostate subcellular fractions to sequester polyamines exerts the following effects: a) minimizes aggregation of most membranous material and lowers its sedimentation rate; b) removes some ribosomes from the microsomal vesicles; c) causes disruption of nuclei and an apparent subnuclear fractionation. 2. 2) After removal of nucleic which interfere in fractionation, heparin was added to subfractionate the other cellular components. Heparin concentration and centrifugation conditions were tested to set up a reliable method of subcellular fractionation. 3. 3) The distribution of the marker enzymes galactosyltransferase (Golgi apparatus) and acid phosphatase (lysosomes and secretory granules) were checked to assess the usefulness of the method.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
