Effect of Hemoperfusion of Cross-Linked Agarose Coated Activatedcharcoal (CAAC-II) on T-Lymphocyte Function in Rats and Guineapigs

Abstract

Blood of rats and guinea pigs on cross-linked agarose coated activated charcoal (CAAC-II) hemoperfusion was analyzed over a follow-up period of 48 hours for the capacity of T-lymphocyte to transform under the stimulation of mitogen (PHA) in vivo and in vitro respectively. In the rate hemoperfusion, the in vivo T-lymphocyte transformation and lymphocyte count of peripheral blood were involved. In guinea pigs, the lymphocyte cultures were labelled with 3H-TdR and the radioactive incorporation was measured on the liquid scintillation counter. Our results revealed that the T-lymphocyte transformation function remained unchanged after 60-minute CAAC-II hemoperfusion although there was a transient and incomplete suppression of the T-lymphocyte transformation function both in vivo and in vitro immediately after the anesthesia and operation with blood vessel cannulation. The peripheral blood lymphocyte count also remained stable during the 60-minute CAAC-II hemoperfusion on rats and guinea pigs. We concluded that the charcoal hemoperfusion can be used as an important method of treatment for drug intoxication, uremia and hepatic coma with no harm to the body's immune function.