Abstract
AbstractBackgroundThe potential effect of increase in hemoglobin (Hb) A2and HbF on glycated hemoglobin (HbA1c) measurements were investigated using a high-performance liquid chromatography (HPLC) method compared with an immunoturbimetric assay.MethodsSamples producing abnormal chromatograms during the measurement of routine HbA1ctesting with HPLC were further analyzed to characterize abnormal Hb variants. Patients were divided into three groups that had only high HbF (group 1), only high HbA2(group 2), and both high HbA2and HbF (group 3). HbA1cvalues of patients were re-assayed using the immunoturbidimetric method (Advia, Siemens Healthcare, Germany).ResultsHbA1clevels were significantly higher in all groups measured by immunoassay than in HPLC. We found a positive correlation between HPLC and immunoturbidimetry in the group 2 and a slight correlation in the group 1. There was no correlation between the two methods in group 3.ConclusionsHbA1cmeasurement by HPLC method interfering with elevated HbA2and HbF, especially HbF, should be verified by an immunoturbidimetric method.
Highlights
IntroductionA normal hemoglobin molecule is composed of two dissimilar pairs of polypeptide chains (alpha and non-alpha) called globins
A normal hemoglobin molecule is composed of two dissimilar pairs of polypeptide chains called globins
We found a positive correlation between high-performance liquid chromatography (HPLC) and immunoturbidimetry in the group 2 and a slight correlation in the group 1
Summary
A normal hemoglobin molecule is composed of two dissimilar pairs of polypeptide chains (alpha and non-alpha) called globins. The problems in β-globin chain production, the production of δ-globin and γ-globins may be increased, resulting in higher levels of HbA2 and HbF. Carriers of β-thalassemia according to World Health Organization estimates are approximately 1%–5% of the world’s population [2] They are a very common patient group in Turkey, because it originated in the Mediterranean and in the Middle East [3]. The potential effect of increase in hemoglobin (Hb) A2 and HbF on glycated hemoglobin (HbA1c) measurements were investigated using a high-performance liquid chromatography (HPLC) method compared with an immunoturbimetric assay. Results: HbA1c levels were significantly higher in all groups measured by immunoassay than in HPLC. Conclusions: HbA1c measurement by HPLC method interfering with elevated HbA2 and HbF, especially HbF, should be verified by an immunoturbidimetric method
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