Abstract
Many reports have shown that patients with Hp-associated chronic gastritis exhibit anxiety and poor sleep quality. However, less is known about the effects and specific manifestations of Hp-associated chronic gastritis on autonomous activity and sleep quality in animals. Here, we investigated the effect of Helicobacter pylori (Hp)-associated chronic gastritis on autonomous activity and sleep quality in mice. To do this, a Hp-associated chronic gastritis mouse model was first established, then analyzed for autonomous activity, relative to controls, for 15 min using an autonomous activity tester. Next, sleep quality of mice was detected by sodium pentobarbital-induced sleep experiment and results compared between groups. The results showed that male mice in the model group exhibited higher activity counts but lower forelimb lift counts, relative to those in the control group, although there were no significant differences (all p > .05). Conversely, female mice in the model group recorded lower activity counts, albeit at no significant difference (p > .05), and significantly lower counts of forelimb lift (p < .05), relative to those in the control group. Notably, male mice in the model group had longer sleep latency and shorter sleep duration than those in the control group, albeit at no significant differences (all p > .05). On the other hand, female mice in the model group recorded significantly longer sleep latency as well as shorter sleep duration compared to those in the control group (all p < .01). We conclude that Hp-associated chronic gastritis exerts certain effects on autonomous activity and sleep quality of mice in a gender-dependent manner. Notably, female mice with Hp-associated chronic gastritis had lower activity and forelimb lift counts, as well as prolonged sleep latency, and shortened sleep duration. These effects were all statistically significant except for activity counts.
Highlights
Chronic gastritis is a chronic inflammatory disease of the gastric mucosa characterized by various etiologies (Spużak et al, 2020), one of which is Helicobacter pylori (Hp) (Nagy et al, 2016; Kalach et al, 2021)
In terms of the weight of all mice at the end of the study, as shown in Figure 1, there was no significant difference between the experiment group and the control group at the beginning and end (p > .05)
Among four mice that were randomly selected for gastric tissue collection after quarantine, 1 was eliminated due to improper anatomical operation, while the remaining three were negative for Hp following polymerase chain reaction (PCR) and urease test (Figure 2A)
Summary
Chronic gastritis is a chronic inflammatory disease of the gastric mucosa characterized by various etiologies (Spużak et al, 2020), one of which is Helicobacter pylori (Hp) (Nagy et al, 2016; Kalach et al, 2021). Previous studies have shown that prevalence, grade and severity of chronic gastritis increases with the degree of Hp infection (Tiwari et al, 2020; Chitapanarux et al, 2021). Effect of Hp in Mice clinical studies have shown that patients with Hp-associated chronic gastritis exhibit anxiety and poor sleep quality (Buzás, 2006; Takeoka et al, 2017; Kim et al, 2020), but the specific characteristics and pathological mechanisms remain unclear. The effects and specific manifestations of Hpassociated chronic gastritis on autonomous activity and sleep quality in animals remain unclear, due to scarcity of specific experimental studies. We adopted a previously established Hp-associated chronic gastritis mouse model (Lee et al, 1997) to explore the effect and specific manifestations of Hp-associated chronic gastritis on autonomous activity and sleep quality in mice. We verified whether the observed effects were consistent with that in the human body, with the aim of laying a foundation for future experimental studies
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