Abstract
The aim of this study is to investigate the effects exerted by He:Ne laser (632.8 nm) low level laser irradiation (LLLI) on neutrophil activity in response to op sonized zymosan. Human buffy coat leukocytes primed with opsonized zymosan were exposed to 10 mw He:Ne laser at energy densities of 1-5 J/cm 2 . Phagocytic activity was assessed by measuring percent of phagocytosis, H2O2 and nitric oxide (NO) generation together with neutrophil-associated nitric oxide synthetase activity (NOS) and Arginase I mRNA. LLLI stimulated neutrophil's phagocytic activity and free radicals generation. This effect was intensified, when neutrophils were dually stimulated by LLLI and opsonized zymosan within the dose range of 3-5 with maximum activity at 5 J/cm 2 .
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