Effect of Growth Temperature on the Production of Bacterial .BETA.-Lactamase in Yeast Cells.

Abstract

The stability of bacterial β-Lactamase (bla) in Saccharomyces cerevisiae containing YEp vector was monitored at different temperatures. It was found that in all runs the loss of bla activity occurred during the late logarithmic-growth phase. This loss was activated at relatively high temperatures. A transient decrease in cell doublings was observed at the beginning of loss of bla activity, which is followed by approximately one doubling before the end of batch growth. This would appear to indicate that the loss of bla activity is closely related to growth regulation mechanisms of S. cerevisiae cells.