Abstract
Fatty acids occurring in lipids of Corynebacterium cyclohexanicum are 12-mefhyltridecanoic, myristic, 13-methyltetradecanoic, 12-methyltetradecanoic, n-pentadecanoic, 14-methylpentadecanoic, palmitic, 15-methylhcxadecanoic, and 14-methylhexadecanoic (in order of increasing retention times) identified by gas–liquid chromatographic retention characteristics and mass spectrometry. In glucose-grown cells, 12-methyltetradecanoic (35%) and 14-methylpentadecanoic (35%) acids were major acid components, whereas in the cells grown on cyclohexanecarboxylic, m-hydroxybenzoic, butyric, and acetic acids, 12-methyltetradecanoic acid was always the major cellular fatty acid, constituting 65–81% of the total fatty acids. The phase transition temperature of phospholipid sample from cells grown on glucose (22 °C) was higher than the corresponding values with butyrate (10 °C) and acetate (13 °C). The upper limit of growth temperature, however, was no different between cells grown on butyrate or glucose.
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