Effect of Growth Regulators on Rooting of Carnation (<i>Dianthus caryophyllus</i>) Tissue Culture Seedlings

Abstract

In order to understand the effects of different growth regulators and their concentrations on the rooting culture of carnation tissue culture seedlings, the optimal growth regulators and their concentrations were explored by adding different concentrations of IAA and IBA to the basic culture medium for tissue culture experiments and observing the rooting and growth of carnation tissue culture seedlings. The results showed that the sterilization effect of 10% sodium hypochlorite for 4 min was the best; the root length and number of roots in MS medium were significantly higher than that in 1/2 MS and 2 MS medium, and the roots were faster, the rooting rate was high, and the plant growth was good; IAA and IBA of 1.0 mg/L were all conducive to the root elongation, and significantly increased the number of roots, the rest of the concentration promoting effect was not significant, and the high concentration would inhibit the root growth. The growth of the plants treated with IBA was better, and the best was 1.0 mg/L IBA, which was most conducive to the rooting culture of carnation. The above results provide further theoretical basis for in vitro rapid propagation and industrial production of carnation cut flowers.