Effect of Growth Regulators and Thiamine – HCL on in vitro Propagation of Rose (Rosa hybrida L.) cv. “Sarra” Using Axillary Buds.

Abstract

This study was carried out to determine the optimum concentrations of the growth regulators benzyl adenine (BA) and naphthalene acetic acid (NAA) alone and in combination, and Thiamine–HCl (vitamin B 1 ) for in vitro propagation of ( Rosa hybrida L. ) cv. “Sarra” . Axillary buds explants were cultured on Murashige and Skoog m e d i u m a n d incubated at 20±/1°C and photoperiod of 16/8 h light/darkness. A f t e r s i x w e e k s , results showed that using 0.3 mg/l of the growth regulator BA, was the best concentration for shoot growth and development. Best rooting was obtained when using 0.3 mg/l of the growth regulator NAA. Adding each growth regulator alone gave better results than adding them in combination for both rooting and shoot growth. Thiamine–HCl proved to be an essential element for in vitro propagation of rose ( R. hybrida) cv. “Sarra” at concentration of 0.8 mg/l which gave the highest growth rates of the in vitro propagated plantlets.