Effect of Growth Hormone and Glucose on 14C Incorporation by the Perfused Dog Pancreas

Abstract

SummaryThe isolated, recirculated, perfused dog pancreas has been used to study the effect of elevated glucose concentrations and GH on the incorporation of 14C labeled amino acids into the insulin containing fraction. The greatest incorporation occurred at the low glucose level. High glucose levels alone and 1 or 3 units of GH at low or high glucose levels caused a reduction of 14C amino acid incorporation into the IRI containing fraction below that seen with low glucose levels alone. Pretreatment of the pancreas donors with GH restored the 14C incorporation to that seen with low glucose alone. The results are discussed.