Effect of Grape Seeds Powder on the Level of Some Antioxidants and Lipid Peroxidation of White Male Albino Rats Exposed to Oxidative Stress

Abstract

The present study included the investigation of the effect of grape seeds powder Vitis vinifera in preventing oxidative stress induced by hydrogen peroxide H2O2 at a concentration 0.5 % given with consumed drinking water in male albino rats Rattus norvegicus aged 2.5 – 3 months. Rats were randomly divided into 5 groups (5 rats/group) and the rats were treated with forage to which grape seeds powder were added at different concentrations 200, 400, 600 mg/kg. B. Wt. of grape seeds powder daily for eight weeks. The results showed that treatment with 0.5% H2O2 caused a significant increase in malondialdhyde (MDA), bilirubin and uric acid level and a significant decrease in glutathione (GSH), albumin level and the enzyme activity of superoxide dismutase (SOD) in the serum of treated rats compared with control group, also the above changes indicating the ability of H2O2 to induce the oxidative stress in albino rats. The results also showed that treatment with grape seeds powder caused a significant decrease in MDA, bilirubin and uric acid level and a significant increase in GSH, albumin level and the activity of SOD compared with control. The results also showed the decrease and increase were more prominent in rats treated with 600 mg /kg. B. Wt. of grape seeds powder, these results indicate the protective effect of grape seeds powder against oxidative stress.