Abstract
Two experiments were designed to study the effect of semen extenders and freezing regimens on post-thaw semen motility and viability index, effect of bucks breed was also included (experiment 1). In experiment 2, semen diluents effects on reproductive performance was conducted. Semen was collected from bucks breeds (Aradi (A), Damascuss (D) and cross (½A½D). Good quality semen was divided into 4 portions, each diluted with one diluent (Milk, Na.Citrate, Tris and Na.Bicarbonate). The diluted semen was packaged into 0.5ml straws then cooled to 5°C. After equilibration, half of the packaged straws were suspended 15cm above liquid nitrogen (LN) for 15min (Freezing regimen1; slow). Other half of straws was suspended at height 15 and 5cm of LN for 10 and 5min, respectively (Freezing regimen2; rapid) before plunged into LN. Frozen semen was thawed for post-thaw motility and viability. In the second experiment, semen with good quality was extended with three types of extenders (Milk, Na.Citrate and Tris). Diluted semen were cooled to 5°C and used for AI. Results revealed that, pre-freeze semen motility was significant higher in Tris, Na.Citrate and Na.Bicarbonate than milk diluent. Post-thaw semen motility and viability were highly significant for milk and Na.Citrate than Tris and Na.Bicarbonate diluents. Post-thaw semen motility was significantly higher in Aradi and Damascus than cross breed. Post-thaw semen motility and viability revealed, significant higher means for Freezing regimen 2 than regimen 1. Milk was significantly higher than Tris and Na.Citrate diluents for fertility and fecundity. It was concluded that, regarding to post-thaw semen viability, fertility%, kidding%, fecundity% and prolificacy%, milk is preferable than Tris and Na.citrate diluents.
Highlights
The success of artificial insemination (AI) is based on the ability to efficiently collect and cryopreserve spermatozoa from quality bucks for use on does over generations (Amoah and Gelaye, 1990)
Salvador et al (2007) cited that, milk diluent provided higher viability of spermatozoa than semen diluted in Tris and citrate
The present study aimed to investigate the effect of semen extenders, freezing regimens and bucks breed on postthaw semen motility and viability index, as well as investigate if semen diluents influenced reproductive performance of goats in arid environment
Summary
The success of artificial insemination (AI) is based on the ability to efficiently collect and cryopreserve spermatozoa from quality bucks for use on does over generations (Amoah and Gelaye, 1990). The effect of semen extenders, freezing regimens and of bucks breed on post-thaw semen motility and viability index were conducted in this experiment. The viability index of post-thaw semen motility were significant higher (P
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