Effect of GnRH administration, combined with the ram effect, on the occurrence of ovulation and pregnancy during the transition period from anoestrus in crossbred ewes

Abstract

The effect of a GnRH analogue (buserelin) combined with the ram effect on the reproductive efficiency of ewes was investigated in 105 cross-bred fat tailed ewes, during the transition period from anoestrus to the natural breeding season. Plasma progesterone concentration was used in the assessment with regard to ovulation and pregnancy. Ewes were maintained on natural pastures composed of medium to low quality forages, and received supplementation (40% alfalfa hay: 60% wheat straw) ad libitum, plus 100–300g barley grain per head per day. Ewes were isolated from the rams for at least two months and then kept in close proximity of the rams for one week, before the introduction of the rams. The ewes were randomly divided into three equal groups (n=35 per group). With the introduction of the rams into the flock (day 1) one group was considered as the control and the other two groups were treated with 4.2μg (low dose) and 8.4μg (high dose) buserelin on days 5 and 19, respectively. Blood samples were collected on days 5, 12, 26 and 120 after ram introduction for determining the plasma progesterone levels. On day 12 (one week after treatment) the high dose GnRH group recorded significantly lower plasma P4 concentrations (P<0.05), compared with the control group (1.0±0.1ng/ml versus 2.4±0.4ng/ml). On the same day the low GnRH dose group recorded intermediate P4 concentrations, recording no significant differences with the other two groups. The high dose group recorded a significantly (P<0.05) higher proportion of non-ovulated ewes (61.8%), compared to the control (32.3%) and low dose (31.4%) groups on day 12 of the study. At days 5 and 26 these differences were not significant, but the proportion of non-ovulated ewes was higher in the high dose buserelin treatment group. The percentage of pregnant (plasma P4>2.5ng/ml) and non-pregnant (plasma P4≤2.5ng/ml) ewes at day 120 of the study was not statistically different between the treatment groups. The pregnancy rate was highest in the control group (97.1%), when compared to the treated ewes (94.3% and 88.6% in low dose and high dose treatment groups, respectively). Treatment with buserelin combined with the male effect during the breeding season negatively affected the plasma P4 concentration, reducing the reproductive performance of the ewe treatment groups.