Abstract
1. 1. Fatty acid synthesis in cow and rat mammary tissue was compared utilizing tissue slice incubations. 2. 2. Lipogenesis occurred in cow tissue with acetate as the only substrate, but was negligible in rat tissue. 3. 3. Glucose additions stimulated lipogenesis from acetate 3-fold in cow and 115-fold in rat tissue. 4. 4. In the presence of glycerol, acetate incorporation into fatty acids represented 63 and 2 per cent of the rate found with glucose additions for cow and rat, respectively. 5. 5. Species differences noted were consistent with ruminant mammary tissue having alternate means of generating a portion of NADPH from acetate, presumably via NADP-isocitrate dehydrogenase.
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