Effect of glutathione S‐transferase M1 and Tl polymorphism on the level of plasma antioxidant vitamin in Korean subclinical hypertensive patients after kale juice supplementation (1035.9)

Abstract

Glutathione s‐transferase (GST) forms a multigene family of phase II detoxification enzymes which are involved in the detoxification of reactive oxygen species. In this study, whether daily supplementation of kale juice could modulate levels of plasma antioxidant vitamin, and whether this modulation could be affected by GSTM1 and T1 polymorphisms was examined. Eighty four subclinical hypertensive patients showing systolic blood pressure (BP) over 130 mmHg or diastolic BP over 85 mmHg received 300 ml/day of kale juice for 6 weeks, and blood samples were drawn before and after these 6 weeks. The plasma level of vitamin C increased significantly in the GSTM1‐null genotype, but not significantly in the wild type. However, the level significantly increased in both null and wild GSTT1 genotypes. The plasma level of β‐carotene increased in both null and wild genotypes of GSTM1 and GSTT1 after the kale juice supplementation. The plasma levels of retinol, α‐, γ‐tocopherol, α‐carotene, cryptoxanthin, and coenzyme Q10 did not show any significant difference in either GSTM1 or GSTT1 genotypes and in null and wild genotypes. This study suggests that the polymorphism of the GSTM1 gene exists in the plasma level of vitamin C in subclinical hypertensive patients (This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) under the sponsorship of the Ministry of Education, Science and Technology (2012‐0001682)).