Abstract
Effect of Glutaraldehyde on the Conformational Properties of Kluyveromyces lactis β-Galactosidase and Its Application to Sucrose Biosensor Preparation
Highlights
The effect of glutaraldehyde on the conformational properties of Kluyveromyces lactis β-galactosidase was determined
The purpose of this paper is to study the effect of glutaraldehyde on the conformational properties of Kluyveromyces lactis β-galactosidase by fluorescence spectroscopy[19,20,21] and its application to sucrose biosensor preparation
An interesting phenomenon was first found, that is, the optimum substrate of β-galactosidase from Kluyveromyces lactis interchanged from lactose to sucrose when it reacted with 5% glutaraldehyde for 80 min
Summary
The effect of glutaraldehyde on the conformational properties of Kluyveromyces lactis β-galactosidase was determined. Its mechanism was studied by detecting the changes in fluorescence absorption This is the first discovery that β-galactosidase can be converted to another kind of enzyme which can catalyze sucrose to produce glucose rather than lactose. The sucrose biosensor exhibited excellent diffusion and stability, and retained its sensitivity after 7 d of continuous use This sucrose biosensor is expected to provide an effective and economical alternative for the quick and sensitive detection of sucrose in the food and beverage industries in the future. What is peculiar is that the β-galactosidase from Kluyveromyces lactis loses its lactose-hydrolyzing activity when in contact with a certain concentration of glutaraldehyde after a period of time, it will gain the ability to hydrolyze the sucrose, which is similar to lactose
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