Abstract
Embedding without dehydration in a polymerizable mixture of glutataldehyde and urea was tested on skeletal muscle and myocardium prepared in various ways. This method, which theoretically should decrease electrolyte disturbances, appears preferable to conventional techniques for electron microscope X-ray microanalysis and histochemical studies. Analysis of minimally treated tissues showed that chlorine and calcium are easily detectable as intracellular elements when precipitated with silver and antimony, respectively. Glutaraldehyde + urea embedding proved visually and analytically superior to Epon in preserving ionic stability. An experimentally produced increase in intracellular myocardial calcium was also better reflected by this method. Several points of interest relating to nuclear calcium shifts in the myocardium were noted.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
