Effect of ginsenoside Rb1 on bioactivity of olfactory ensheathing cells

Abstract

Objective To investigate influence of ginsenoside Rb1 on the proliferation and bioactivity of olfactory ensheathing cells (OECs). Methods OECs were primary cultured and purified from olfactory bulb of the adult SD rats. MTT assay was used to detect proliferation of OECs treated with ginsenoside Rb1 (intervention concentrations of 0, 10, 20, 40, and 80 μg/ml and intervention time of 12, 24, 36, 48, and 60 hours). Optimal concentration and intervention time of ginsenoside Rb1 was determined and performed in the succedent experiments.Purified cells were divided into blank control group and ginsenoside Rb1 group. RT-PCR was utilized to determine mRNA expressions of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial derived neurotrophic factor(GDNF) and neural cell adhesion molecule(N-CAM) in the two groups. ELISA analysis was performed to measure secretion levels of NGF, BDNF and GDNF in the cultural supernatant. Results MTT analysis suggested ginsenoside Rb1 promoted proliferation of OECs with optimal effect at 20 μg/ml concentration for 48 hours (0. 648±0. 019, P<0. 05). RT-PCR analysis demonstrated that mRNA expressions of NGF, BDNF, GDNF and N-CAM were significantly up-regulated in ginsenoside Rb1 group compared to those in blank control group (0.620±0.011vs 0.180±0.011, 0.511±0.090 vs 0.293±0.051, 0.343±0.042 vs 0.064±0.005, 0.839±0.017vs 0.717±0.044) (P<0.05). ELISA analysis confirmed that secretions of NGF, BDNF and GDNF was increased in Rb1 group compared to those in blank control group (200.167±8.361 vs 51.467±3.815, 156.700±4.190 vs 96.500±2.707, 26.264±5.864 vs 4.917±10.894, P<0.05). Conclusion Ginsenoside Rb1 significantly promotes proliferation and bioactivity of OECs and hence benefits to spinal cord injury repair. Key words: Ginsenosides; Spinal cord injuries; Olfactory ensheathing cells