Abstract
Objective To investigate the neuroprotective effect of ginkgolide B(BN52021)on the histopathology of brain tissue after traumatic brain injury in rats.Methods Forty-eight healthy SD rats,weighing 250g,were evenly randomized into 4groups:sham control group,model group,low dose BN52021group and high dose BN52021group.Rats in the latter 3groups were made into fluid percussion brain injury models.After operation,the rats in the low and high dose BN52021groups were treated with BN52021(low dose:1mg/kg,ip,high dose:5mg/kg,ip,once daily for 7days).On the 7th day after treatment, cerebral tissues were harvested from each group,and the histopathological changes of brain tissue were observed by Fast blue, electron microscope and immunohistochemical method.Results Compared with sham control group,model group had significantly decreased neurons(P0.05),increased OX-42immunoreactive microglial cells and astrocytes(P0.05),and cells positive for caspase-3(P0.05).Electron microscope found chromatin aggregation,nuclear fragmentation,rounder and larger mitochondria,void formation and disappeared cristae of mitochondria,endoplasmic reticulum hypertelorism,increased lysosomes,and nuclear membrane folding.Compared with model group,the low and high dose BN52021groups had significantly decreased proportions of microglial cells and astrocytes(P0.05),significantly decreased caspase-3positive cells(P0.05), and improved ultrastructure,with the improvement in the high dose group being more notable than that in the low dose group. Conclusion BN52021has protective effect on the morphology of brain tissue in rats with traumatic brain injury.
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