Effect of ginkgolide B on expression of Foxg1 gene and proliferation of cells in brain tissue of newborn rats with hypoxic-ischemic brain damage

Abstract

Objective To observe the effects of ginkgolide B (GB) on mRNA expression of foxg1 and proli-feration of cells in brain tissue of newborn rats with hypoxic-ischemic brain damage (HIBD). Methods A total of 128 clean 7-day-old healthy SD rats were randomly divided into sham operation group, the model group, the low-GB dose and the high-GB dose treatment groups.Classic Rice method were used to establish HIBD models in the latter 3 groups.Four hours after operation, and GB in dose of 5 mg/kg and 10 mg/kg was given to rats in the low and the high dose treatment groups by intraperitoneal injection postoperatively, once a day for 5 days, while sham operation and model groups were treated with equal physiological saline.All groups were respectively sacrificed on 3 d, 7 d, 14 d, 28 d respectively.Quantitative real-time fluorescent polymerase chain reaction was employed to detect expression of Foxg1 gene.Then the number of 5-bromodeoxyuridine positive cell in subgranular zone was investigated by immunofluorescent staining. Results The Foxg1 mRNA expression was observed 3 days after HIBD, peaked on 7th day, and then declined gradually; the levels of Foxg1 mRNA in the 2 treatment groups were higher than that of the HIBD group(all P<0.01); The expression of Foxg1 at 7 d, 14 d, 28 d, in high-dose group were higher than those in the low-dose group(all P<0.01). The number of 5-bromodeoxyuridine positive cell was increased after HIBD, and the levels in the low-and the high-dose treatment groups were all higher than that of the model group(all P<0.05); the number of positive cell in high-dose treatment groups were higher than that in the low-dose treatment groups(P<0.05). Conclusions GB can promote the expression of Foxg1 gene and improve the proliferation of cells in Brain tissue after HIBD, which shows more significant efficacy in high-dose group than in low-dose group. Key words: Hypoxic-ischemic brain damage; Ginkgolide B; Foxg1 gene