Effect of germinated brown rice on the LPS‐induced inflammation in adipocytes

Abstract

Germinated brown rice (GBR, Orysa sartiva L.) has been reported to have anti‐obesity and anti‐inflammatory effects. However, the mechanisms underlying the anti‐inflammatory effect of GBR on adipocytes are not fully understood. The aim of this study was to explore the anti‐inflammatory mechanisms of GBR on lipopolysaccharide (LPS)‐stimulated 3T3‐L1 adipocytes. 3T3‐L1 adipocytes were pretreated with GBR extracts (0–20 mg/ml) 1h before LPS stimulation. The mRNA expressions of adipokines and Toll‐like receptor 4 (TLR4) were measured by RT‐PCR. The protein expressions of TLR4‐related molecules were detected by western blotting and nuclear factor‐κB (NF‐κB) activation was measured. Our results showed that GBR extract dose‐dependently inhibited mRNA expressions of LPS‐induced tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6), and monocyte chemoattractant protein‐1 (MCP‐1). GBR extract was found to inhibit LPS‐induced mRNA expression of TLR4 and protein expressions of both myeloid differentiation factor 88 (MyD88) and TNF receptor‐associated factor 6 (TRAF6). Furthermore, GBR extract significantly inhibited extracellular receptor‐activated kinase (ERK) phosphorylation and NF‐κB activation. These results suggest that GBR extract has the anti‐inflammatory property on LPS‐induced inflammation via inhibiting TLR4 signaling including ERK and NF‐κB signaling pathways in adipocytes. (This research was supported by the Soonchunhyang University Research Fund.)This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.