Effect of Geometrical Structure, Drying, and Synthetic Method on Aminated Chitosan-Coated Magnetic Nanoparticles Utility for HSA Effective Immobilization.

Marta Ziegler-Borowska,Anna Kozakiewicz,Kinga Mylkie,Mariana Kozlowska,Anna Ilnicka,Anna Kaczmarek-Kedziera,Pawel Nowak,Dorota Chelminiak-Dudkiewicz

doi:10.3390/molecules24101925

Marta Ziegler-Borowska, Anna Kozakiewicz + Show 6 more

Open Access

https://doi.org/10.3390/molecules24101925

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Journal: Molecules (Basel, Switzerland)	Publication Date: May 18, 2019
Citations: 11	License type: CC BY 4.0

Affiliation: Nicolaus Copernicus University

Abstract

Human serum albumin (HSA) is one of the most frequently immobilized proteins on the surface of carriers, including magnetic nanoparticles. This is because the drug–HSA interaction study is one of the basic pharmacokinetic parameters determined for drugs. In spite of many works describing the immobilization of HSA and the binding of active substances, research describing the influence of the used support on the effectiveness of immobilization is missing. There are also no reports about the effect of the support drying method on the effectiveness of protein immobilization. This paper examines the effect of both the method of functionalizing the polymer coating covering magnetic nanoparticles (MNPs), and the drying methods for the immobilization of HSA. Albumin was immobilized on three types of aminated chitosan-coated nanoparticles with a different content of amino groups long distanced from the surface Fe3O4-CS-Et(NH2)1–3. The obtained results showed that both the synthesis method and the method of drying nanoparticles have a large impact on the effectiveness of immobilization. Due to the fact that the results obtained for Fe3O4-CS-Et(NH2)2 significantly differ from those obtained for the others, the influence of the geometry of the shell structure on the ability to bind HSA was also explained by molecular dynamics.

Highlights

IntroductionEspecially in the design and synthesis of new materials, significantly affects the development of many branches of science and technology [1]
Rapid progress in nanotechnology, especially in the design and synthesis of new materials, significantly affects the development of many branches of science and technology [1]
It is well-known that chitosan coated on a magnetic core keeps free amino groups on the surface [21]

Summary

Introduction

Especially in the design and synthesis of new materials, significantly affects the development of many branches of science and technology [1]. Molecules 2019, 24, 1925 whose magnetic core can be coated with both low- and macro-molecular compounds [2] This coating leads to a material with surface properties designed precisely for its intended use and conducted to the target place, for instance in a living organism, and effortlessly separable from the reaction mixture because of their superparamagnetic properties [3,4]. One of the most common applications of MNPs is a support for the immobilization of catalysts, drugs, and bioligands such as proteins [5,6,7,8] Nanoparticles designed for this purpose must possess either a surface modified for an effective physical adsorption or enriched in reactive groups prone to forming covalent bonds in the case of chemical immobilization of a ligand [9,10,11]

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