Effect of Gel Secretome Hypoxia Mesenchymal Stem Cell on Expression of TGF- β and IL-6 (In Vivo Experimental Study in Male Rats of Wistar Strains Model Hyperglycemic Wounds)

Abstract

Wounds disrupt normal skin structure, function, and shape. The healing process is hampered by hyperglycemia or high blood glucose levels resulting in wounds healing longer, resulting in an extension of the healing phase. The aim of this study was to determine the effect of administering hypoxic MSC secretome gel on the expression of the TGF-β and IL-6 genes in male white Wistar rats with a hyperglycemic injury model. True experimental in vivo research with a Post Test Only Control Group design. Consisting of 5 groups, namely the healthy mice group (K1), the base gel treated mice group (K2), the standard intervention treatment group (positive control) with gentamicin (K3), the secretome treatment and intervention group with a dose of 100 μL (K4) and a dose of 200 μL (K5). The research sample consisted of 30 male Wistar rats. Mouse skin tissue was analyzed on day 46 after termination to see the expression of the TGF-β and IL-6 genes using the quantitative RTq-PCR method. There was a significant difference in TGF-β gene expression between treatment groups using the One-way Anova test 0.024 (p < 0.05), various doses of MSC hypoxic secretome gel had an effect on increasing TGF-β gene expression using Tamhane's Post Hoc test (p < 0.05 ) showed the highest increase at a dose of 200 μL compared to the others, while IL-6 gene expression had a significant difference with the Kruskal-Wallis test 0.001 (p < 0.05), using MSC hypoxia secretome gel had an effect on decreasing IL-6 gene expression with results lowest at a dose of 200 μL. MSC hypoxic secretome gel at a dose of 200 μL/BW effectively increased TGF-β gene expression and decreased IL-6 gene expression in male white Wistar rats with a hyperglycemic injury model.