Abstract
Objective To investigate the effect of systemic administration of gabapentin on the expression of Nrf2 in dorsal root ganglion neurons in a rat model of diabetic neuropathic pain (DNP). Methods 60 male SD rats weighing 200-220 g were randomly divided into 4 groups (n=15 each): control group (group C), DNP group, saline group (SC+ DNP group) and gabapentin group (GBP+ DNP group). Diabetes was induced with streptozocin (70 mg/kg) subcutaneously. Paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured at 1d before and 3, 7, 10, 14, 21, and 28 d after streptozocin injection, and 21 and 28 d after administration of gabapentin (50 mg·kg-1·d-1, once) intraperitoneally. Saline and gabapentin (50 mg·kg-1·d-1) were given intraperitoneally once a day for 7 d starting from 15 d after streptozocin injection in saline and gabapentin groups respectively. The expression of Nrf2 was determined at 21 d after streptozocin injection by immune-histochemistry and Western blot. Results Compared with group C, PWMT(3.95±0.57) was significantly decreased, PWTL(6.3±0.81) was significantly shorten, Nrf2 in expression was significantly decreased in DNP, SC+ DNP and group (P 0.05). Conclusion Systemic administration of gabapentin attenuates diabetic neuropathic pain by enhancing the expression of Nrf2 in DRG neurons. (Chin J Endocrinol Metab, 2018, 34: 951-954) Key words: Gabapentin; Diabetic neuropathic pain; Dorsal root ganglion; Nrf2
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