Abstract
The damping (filtering) action of head space and tubing of a fermenter was estimated. Dissolved oxygen tension in the culture and oxygen concentration in the outlet gas were measured during oscillatory condition and were used to estimate the damping action using fast Fourier transformation. The damping characteristics were found to be equal to a second order low pass filter with a cut off frequency of 1/500 Hz. The damping was found to be dependent on gas flow rate and oxygen concentration in the gas flow. Increasing the gas flow rate increased damping, however, increasing the oxygen content in the gas flow decreased damping. Data reconciliation of undamped oxygen concentrations in the outlet gas was performed using two different approaches.
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