Abstract
Narrow genetic base is a main problem hindering the progress of any crop plant breeding and improvement. The present experiment was designed to create novel variation in snake gourd with the use of varying doses (0, 100, 150, 200, 250 and 300 Gy) of acute gamma irradiation to seeds of cultivar Nilgunj Local and determine LD50 and GR50 values. One-way ANOVA results for all the parameters included in the study except germination shows that the observed averages of treatment levels are significantly different at 1% significance level (p-values < 0.01) and the germination shows significant difference at 5% significance level (p-value=0.0266). LD50 value based on germination percentage was arrived at 181.85 Gy. GR50 values of different seedling parameters of the cultivar were 176.32 Gy for germination percentage, 152.45 Gy for shoot length, 213.25 Gy for root length, 173.45 Gy for number of leaves, 171.86 Gy for fresh weight of seedling and 193.68 Gy for dry weight of seedling. These doses could be used for generating sufficient variation, which can be used in future breeding and improvement of snake gourd.
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