Abstract
BackgroundAdvanced glycation end products (AGE) is a toxic compound in the human body that can deteriorate health and induce an inflammatory response. One of the type of cells affected is Leydig cells, cells that produce testosterone and located in interstitial areas of the testes. Pericarp extract of Garcinia mangostana contains an antioxidant compound called gamma-mangostin that can decrease inflammatory responses and toxic effects of AGE. We aimed to compare the testosterone levels in Leydig cell culture of Sprague-Dawley rat induced by AGE only and following gamma-mangostin.MethodsAn experimental laboratory study was conducted on testosterone level in Leydig cell culture of Sprague-Dawley rats induced by advanced glycation end products 200 μg/mL and given gamma-mangostin 5 μM compared to cell cultures that were not given gamma-mangostin.ResultsNine Leydig cell cultures were ascertained and divided into three groups. No significant difference was found in the testosterone level of Leydig cell culture given AGE only (1.33 ng/105 cells/24 h) compared to the group given AGE and gamma-mangostin (1.30 ng/105 cells/24 h) (p = 0.535).ConclusionThe testosterone level in Leydig cell cultures induced by AGE were lower than those not given, but similar in the AGE-only group and group given gamma-mangostin. The mean testosterone levels in all groups were in the range of expected levels (0.025–15 ng/105 cells/24 h). Further study with larger samples is important to clarify and confirm our findings.
Highlights
Advanced glycation end products (AGE) is a toxic compound in the human body that can deteriorate health and induce an inflammatory response
Association between gamma-mangostin, AGE, and testosterone level Table 1 shows that the average testosterone level is higher in group 2 (Leydig cell + AGE-Bovine Serum Albumin (BSA) cell culture 200 μg/mL) than group 3 (Leydig cell + AGE-BSA cell culture 200 μg/mL + gamma-mangostin 5 μM) and the highest at group 1 with a significant difference (p = 0.036)
Linearity test was done on testosterone level data, showing a significance value of 0.297, which means there is a significant linear relationship between the dependent variables and independent variables (AGE-BSA and gamma-mangostin)
Summary
Advanced glycation end products (AGE) is a toxic compound in the human body that can deteriorate health and induce an inflammatory response. One of the type of cells affected is Leydig cells, cells that produce testosterone and located in interstitial areas of the testes. Pericarp extract of Garcinia mangostana contains an antioxidant compound called gamma-mangostin that can decrease inflammatory responses and toxic effects of AGE. Testosterone is produced by Leydig cells, which are cells located in the interstitial testes, between seminiferous tubules. Several studies show that male reproductive health problems are the most common cause of couples with infertility cases. It is known that 30% of all cases of infertile couples are caused by men and tend to increase with older couples to 40% [4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
