Abstract
Abstract In the present study, gamma irradiation was applied to promote the mechanical properties of enzyme- mediated in situ forming hydrogels prepared with tyramine-functionalized gum tragacanth (TA-GT). For this purpose, after gamma irradiation of powder or hydrocolloid solution of gum tragacanth (GT), the physiochemical and rheological properties of GT solution, and resultant hydrogel was investigated. In situ forming hydrogels were prepared via horseradish peroxidase catalyzed coupling reaction of TA-GT in the presence of hydrogen peroxide. Gamma irradiation led to a decrease in GT molecular weight and solution viscosity. Also, the solubility of GT improved and the separation of water soluble/swellable part of gum samples became easier, using gamma irradiation. In addition, by gamma irradiation of GT powder at doses of 5–15 kGy, a polymeric solution with higher concentration could be prepared that resulted in the promotion of hydrogels storage modulus. Further increase of irradiation dose did not improve storage modulus due to the extra decrease of gum molecular weight.
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