Abstract
Objective : Gamma radiation induces free radicals with a corresponding alteration in the cell’s antioxidant defense system. The present study thus aimed at determining the role of gamma irradiation in improving the cosmeceutical potential of CEK in terms of antioxidants.
 Methods : C.emersonii KJ725233 was subjected to low (100 Gy) and high (1000 Gy) gamma irradiation doses and its effect on the chlorophyll content was evaluated. The quantitative alterations in the antioxidant content of CEK were evaluated by phosphomolybdenum assay (TAC), ferric reducing antioxidant potential (FRAP), 2, 2 - Dipheny-1-picryl hydrazyl radical scavenging assay (DPPH), total phenolic (TPC) and total flavonoid content (TFC). Also, the corresponding qualitative alterations in the bioactives of CEK were determined by GC-HRMS analysis. 
 Results : A 179.57±2.55% increase in the total chlorophyll content alongwith a 71.76±2.96%, 32.08±2.16%, 11.67±0.89%, 42.85±8.0% and 31.37±3.18% increase was observed in the TAC, FRAP, DPPH radical scavenging, TPC and TFC was observed in CEK irradiated at 1000 Gy. GC-HRMS analysis revealed the induction of Vitamin E on irradiation at both the doses with a corresponding decrease in the phytol content whereas 100 Gy stimulated the induction of phytosterols.
 Conclusion : The potent intrinsic antioxidant activity of cosmeceutically significant CEK can be elevated with the induction of the most sought after antioxidant in cosmetology – Vitamin E on irradiation.
Highlights
Cultural milieus such as the temperature, light intensity, photoperiod, irradiation and pH are known to impact the growth and subsequently the biochemical composition of microalgae [1]
The plant grade chemicals required for BG-11 medium along with Dipheny-1-picryl hydrazyl radical scavenging assay (DPPH), folin-ciocalteau reagent were procured from HiMedia, Mumbai
The quantitative shift in the total chlorophyll content in C. emersonii KJ725233 (CEK) on irradiation was determined spectrophotometrically and an increase of 179.57±2.55% was observed in CEK irradiated at 1000 Gy
Summary
Cultural milieus such as the temperature, light intensity, photoperiod, irradiation and pH are known to impact the growth and subsequently the biochemical composition of microalgae [1]. Under such constraints, microalgae are reported to produce a maximal amount of varied bioactives as compared to normal conditions due to the stressed altered metabolic pathways. The induction or overproduction of these bioactives is primarily a survival technique to overcome the inconsiderate growth environments [2]. The cell’s antioxidant defense systems have to be modified to balance the detrimental effects of the free radicals generated [6, 7]. Gamma irradiation with Co60 (1.33 MeV) has an ability to induce cytological, anatomical, biochemical, morphological and physiological alterations in cells and tissues [8,9,10,11]
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