Abstract
The demand of grape in Bangladesh is fulfilled through import from foreign countries. The fruits of local cultivars of grapes are sour and seeded. Development of seedless grape varieties having increased sweetness, higher yield with better nutritional quality is necessary to reduce the import dependency. The present research activities are the part of a grape improvement project. A pot experiment was conducted at the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, during June to November 2011 to determine the suitable gamma irradiation doses on growth, leaf area and biochemical characters of grape saplings. Three vegetative bud stages viz. bud initiation stage, 4-leaf stage and 8-leaf stage, and four doses of gamma irradiation viz. 0, 5, 10, and 15 Gy were used as treatments. The experiment was laid out in a Randomized Complete Block Design with four replications. Different irradiation doses and vegetative bud stages showed significant variations in respect of plant growth characters, leaf area, soluble protein and total sugar content. Interaction effects also had significant variations on most of the parameters studied. Higher doses of gamma irradiation had showed detrimental effect on grape saplings. Generally, increased in irradiation doses showed decreased and detrimental effects on most of the parameters under study. Maximum numbers and length of roots, total dry matter, leaf area and chlorophyll-a and chlorophyll-b content were found at 5 Gy irradiation dose. Total soluble protein and sugar content of leaf were found maximum at no irradiation and 15 Gy, respectively. Higher number of roots and length, total dry matter, leaf area, chlorophyll-a, and b and soluble protein content of leaf were observed at bud initiation stage while 8-leaf stage showed maximum total sugar of leaf. In the combined effect of gamma irradiation and vegetative bud stages, all parameters showed best results in 5 Gy with bud initiation stage except total sugar content of leaf.
Highlights
Grape (Vitis vinifera L.) belongs to the family Vitaceae distributed widely all over the world [1] and originated in West Asia [2]
In the present study different doses of gamma irradiation were applied to grape saplings at different growth stages of saplings to determine the suitable gamma irradiation doses on the growth characteristics and better leaf area and biochemical features of grape vines
The cutting plants were replaced in polybag and kept under shade for establishment of plants. Three stages such as bud initiation stage, 4-leaf stage and 8-leaf stage before applying gamma irradiation were prepared by detopping the branches several times
Summary
Grape (Vitis vinifera L.) belongs to the family Vitaceae distributed widely all over the world [1] and originated in West Asia [2]. Grape occupies the first position among the fruits in the world in terms of area and production [3]. In Bangladesh, the requirement of grape was fulfilled through the import from other countries using hard earned foreign currencies as the demand is increasing with the increase in population. Though the grape plants can grow well in Bangladesh environmental conditions, the quality of fruits was not good at all. Recent studies showed that grape could be successfully grown if good variety is chosen and cultural practices are followed [5]. There exists a great shortage of fruit production to fulfill the requirement of the country In such a situation the production of grapes in the country will help to minimize the fruit shortage as well as to reduce the wastage of foreign currency required to import this highly demanded fruit. In the present study different doses of gamma irradiation were applied to grape saplings at different growth stages of saplings to determine the suitable gamma irradiation doses on the growth characteristics and better leaf area and biochemical features of grape vines
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