Abstract
The present investigation was carried out to study the effect of LC50 of the entomopathogenic fungi (EPF), Paecilomyces lilacinus and Beauveria bassiana, on larval mortality of the greater wax moth (GWM), Galleria mellonella L., under laboratory conditions and also to study the effect of different doses of gamma irradiation (70, 100, 125, and 150 Gy), separately or combined with the LC50 of the isolates of the EPF, B. bassiana, and P. lilacinus, on the second-instar larvae of G. mellonella larval mortality. The combined treatment of gamma irradiation and EPF increased the larval mortality rates than that at each treatment alone. The highest percentage of larval mortality was 78 and 84%, with 125 Gy + B. bassiana in the case of F1 male and F1 female, respectively. According to the obtained results, the gamma irradiation increased the pathogenicity of the fungi against the tested larvae. The combination between the two control tools may provide satisfactory control of the insect-pest, especially, in the storage.
Highlights
The greater wax moth (GWM), Galleria mellonella L. (Lepidoptera: Pyralidae), is one of the most devastating and economically important pests of bee wax in the world (Haewoon et al 1995)
Combs in weak or dead colonies and in storage areas are subject to attack (Caron 1992).Chemical pesticides have been the practical method used by growers for the control of economically insect pests, but their negative side-effects on non-target organisms, groundwater contamination, residues on food crops, and the development of insect resistance to chemicals have forced the industry and scientists to focus on developing alternative control measures
Latent effect of gamma irradiation on the mortality of F1 GWM, larvae descendant from irradiated parents Data presented in Table 1 show the effect of gamma irradiation on the percentage larval mortality in the F1 progeny of G. mellonella descendant of the irradiated parental males and females, as full-grown pupae, with the four doses 70, 100, 125, and 150 Gy
Summary
The greater wax moth (GWM), Galleria mellonella L. (Lepidoptera: Pyralidae), is one of the most devastating and economically important pests of bee wax in the world (Haewoon et al 1995). Combs in weak or dead colonies and in storage areas are subject to attack (Caron 1992).Chemical pesticides have been the practical method used by growers for the control of economically insect pests, but their negative side-effects on non-target organisms, groundwater contamination, residues on food crops, and the development of insect resistance to chemicals have forced the industry and scientists to focus on developing alternative control measures. This pest species has received more attention as a model organism for toxicological investigations involving entomopathogens than as a honeybee pest, with more focus. The aim of the present study was to evaluate the effect of the gamma irradiation and/or EPF, separately or combined in controlling G. mellonella under laboratory conditions
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