Effect of Galardin and its Solvents on the Microtensile Bond Strength of Different Adhesive Systems to Dentin

Mohammad Esmaeel Ebrahimi Chaharom,Mahmoud Mahmoud Bahari,Sepideh Golbaz,Mahdi Rahbar

doi:10.4034/pboci.2019.191.12

Mohammad Esmaeel Ebrahimi Chaharom, Mahmoud Mahmoud Bahari + Show 2 more

Open Access

https://doi.org/10.4034/pboci.2019.191.12

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Abstract

Objective: To evaluate the effect of a synthetic inhibitor of MMPs (Galardin) and its solvents [ethanol and dimethyl sulfoxide (DMSO)] on the microtensile bond strength (µTBS) of adhesive systems to dentin. Material and Methods: Sound human third molars (n=180) were randomly assigned into 5 based on solution type: DMSO; ethanol; Galardin + DMSO; Galardin + ethanol; and distilled water as control. Then were further subdivided into 6 based on the adhesive system, i.e. 3-step and 2-step etch-and-rinse (ER), one-step and 2-step self-etch (SE) and universal in ER and SE strategies. The samples underwent a 500-round thermocycling procedure at 5±5/55±5°C and were sectioned into 1-mm2 pieces perpendicularly in a cutting machine. The µTBS was measured at a strain rate of 1 mm/min. Data were analyzed with two-way ANOVA and post hoc Games-Howell tests (p<0.05). Results: The adhesive system and the solution had significant effects on the µTBS (p<0.001). The universal adhesive in the SE mode resulted in a significant decrease in µTBS compared to the other adhesives (p<0.05). Ethanol, too, resulted in a significant decrease in µTBS compared to other solutions (p<0.05). Conclusion: Galardin and its solvents, except for ethanol, had no detrimental effects on the immediate µTBS.

Highlights

Complete penetration of resin into the network of collagen fibers is necessary in order to form a hybrid layer and achieve successful bonding to dentin
Several studies have shown that the integrity of the collagen matrix of dentin in the hybrid layer is a key factor for the longevity of bonded restorations [1,2]
Two-way ANOVA showed that the type of irrigation solution and the type of the adhesive system significantly affected the μTBS (p

Summary

Introduction

Complete penetration of resin into the network of collagen fibers is necessary in order to form a hybrid layer and achieve successful bonding to dentin. Several studies have shown that the integrity of the collagen matrix of dentin in the hybrid layer is a key factor for the longevity of bonded restorations [1,2]. The great resistance of the collagen matrix of dentin against heat and proteolytic agents is attributed to the high rate of intermolecular connections and the strong mechanical structure of this specific connective tissue [3,4]. It has been shown that the collagen matrix and gelatinolytic activity of human dentin matrix is inhibited by protease inhibitors [8,9,10]. One of these agents, Galardin (Ilomastate, GM6001), inhibits the protease activity. Galardin is the specific inhibitor of MMPs, introduced by Grobelny, and has an innate inhibitory activity on MMP1, 2, 3, 8 and 9 [11,12]

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