Abstract
Fucoidan is a fucose-containing sulfated polysaccharide from brown algae such as Fucus vesiculosus, Ecklonia kurome, and Cladosiphon okamuranus. To observe the effects of fucoidan on the differentiation of 3T3-L1. 3T3-L1 preadipocytes were cultured and then divided into 5 groups into whose media were added fucoidan of the concentrations of 0, 10, 50, 100, and 200 ppm, 10 μg/mL insuiln, 0.5 mM 3-isobutyl-1-methylxanthine, and 0.25 μM Dexamethasone on a 60 mm dish. The accumulation of lipid in the cytoplasm of differentiated adipocytes was observed by oil red O staining. There were less and smaller lipid droplets in the 3T3-L1 adipocytes treated with fucoidan as compared with the untreated control (P<0.001). Reverse transcription polymerase chain reaction (RT-PCR) was used for measurement of obesity related genes. RT-PCR analysis revealed that expression level of C/EBPa mRNAs inhibited with fucoidan treatment from 0 to 200 ppm in a dose-dependent manner. Fucoidan decreased the accumulation of lipid drop, and inhibit the terminal differentiation of adipocyte. Fucoidan might be associated with its effect on decreasing the expression of obesity related genes mRNA. These data are suggesting that fuoican has advantages in the treatment of obesity patients with type 2 diabetes.
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