Effect of fucoidan on fibroblast growth factor-2-induced angiogenesis in vitro

Abstract

Fucoidans are sulfated polysaccharides extracted from brown marine algae. A purified fucoidan fraction exhibits the same venous antithrombotic activity as heparin in rabbits, but with a lower anticoagulant effect. Because of its heparin-like structure, we postulated that fucoidan might modulate heparin-binding angiogenic growth factor activity. We thus studied its effect, at antithrombotic concentrations, on fibroblast growth factor (FGF)-2-induced proliferation and differentiation of human umbilical vein endothelial cells. The fucoidan effect on endothelial cell differentiation was evaluated by studying the expression of surface proteins (i.e. integrin, adhesion molecule) known to be modulated by FGF-2 and involved in angiogenesis, and by quantifying closed areas delimited by vascular tubes formed on reconstituted basement membrane. Fucoidan had no modulatory effect on the mitogenic activity of FGF-2, but significantly increased tubular structure density induced by FGF-2. Fucoidan alone increased α 6 integrin subunit expression with only partially organized tubular structure. In the presence of FGF-2, fucoidan enhanced α 6, β 1 and PECAM-1 and inhibited α vβ 3 integrin expression. Heparin had no effect in these systems. The most striking effect of fucoidan was observed on α 6 expression and tube formation was abolished by monoclonal anti-α 6 antibodies. Fucoidan plus FGF-2 effect on α 6 expression was markedly decreased by monoclonal anti-FGF-2 antibodies, indicating that fucoidan acts mainly via FGF-2. These results show that, at antithrombotic concentrations, contrary to heparin, fucoidan can enhance vascular tube formation induced by FGF-2 with a modulation of the expression of surface proteins (mainly α 6) involved in angiogenesis.