Abstract
The effect of freezing and duration of storage of frozen soil on free amino acids and sugars was evaluated. Freezing caused a marked increase in the total amount of free amino acids and sugars extracted. The level of free amino acids and sugars, in frozen soil, remained fairly constant up to a storage period of 6 weeks, after which a prominent decrease occurred. Essentially there was no difference between single freeze-thaw and multiple freeze-thaw cycles. In general, soil respiration and dehydrogenase activity paralleled the level of extractable free amino acids and sugars.
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