Abstract
Effect of four materials including glycine, arginine, proline and sodium glutamate on survival of Bifidobacterium bifidum during freeze-drying was studied by measuring the viable counts of before and after freeze-drying. The addition of glycine, arginine, proline and sodium glutamate (%, w/v) were all 1.0%, 2.0%, 3.0%，4.0% and 5.0%. Results were as follows: addition of glycine, arginine, proline and sodium glutamate into B. bifidum paste could significantly improve the survival rate of B. bifidum and the number of viable cells of lyophilized powder. The optimal concentrations of glycine, arginine, proline and sodium glutamate were 4.0%, 4.0%, 3.0% and 2.0%, the survival of B. bifidum was 87.49%, 84.28%, 67.50% and 69.01%, respectively.
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