Effect of Follicle Diameter and Culture Medium on The in Vitro Maturation of Sheep Oocytes Using Culture Media Supplemented With Different Concentrations of Sucrose in Local Sheep (Ovis Aries)

Abstract

The current study was conducted in the postgraduate laboratory of the college of Agriculture/University of Al-Muthanna/Department of Animal Production, which aimed to find out the effect of follicle diameter and the culture medium on the percentage of in vitro maturation of sheep oocytes (Ovis Aries). The experiment included in vitro maturation of sheep oocytes aspirated from large, medium and small follicles in different culture media (named A, B and C). In vitro maturation of oocytes was carried out using three culture media that differed only in the concentration of sucrose, which were 0. M, 0.25 M and 0.5 M for A, B and C culture media respectively. The results of the current study showed significant superiority (P ≤ 0.05) of culture medium C than the two media A and B in the percentages of in vitro maturation, which were to 38.0 ± 1.71 %, 27.37±1.47 % and 21.902±0.76 % for C, A and B respectively. The results also indicated significant effect (P ≤ 0.05) of follicle diameter in the percentages of in vitro maturation of sheep oocytes which were 38.0±1.71 %, 29.57±2.06 % and 18.5± 0.27 % for large, medium and small follicles respectively. It could be conclusion from the present study that the follicle diameter and culture medium had a significant effect on the percentage of in vitro maturation of sheep oocytes.